The midfielder picked up a serious knee injury while warming up in Italy training, leaving the Scudetto champions without another key star
Juventus will be without Claudio Marchisio for the rest of the season after the midfielder injured a cruciate knee ligament on Friday with the Italy squad.
The Italian FA (FIGC) had confirmed earlier in the day that the 29-year-old would miss Saturday's Euro 2016 qualifier with Bulgaria, with reports suggesting the injury was serious.
Those initial fears have proven accurate, dealing a potentially huge blow to Juve's trophy hopes as they are now without Marchisio until 2015-16 and Paul Pogba until mid-May.
"While warming up the player was suspected to have suffered just a sprain to his right knee," Italy doctor Enrico Castellacci said in a statement on the FIGC's official website.
"An MRI scan was performed immediately, however, and it showed he had ruptured his anterior cruciate ligament.
"After consulting with the medical staff of Juventus, it was decided to release the player to his club for treatment."
Anterior cruciate ligament injuries usually take at least half a year to recover from, putting Marchisio's comeback date towards the end of 2015.
Although there is no international tournament that he will miss out on in the summer, his injury could deal a blow to Juventus' hopes of winning the treble.
They are currently 14 points clear in Serie A, but they are 2-1 down ahead of the away leg in their Coppa Italia semi-final against Fiorentina and face a testing clash with Monaco in the Champions League quarter-finals.
